Analysis of IgM, IgA, and IgG isotype antibodies Directed against SARS-CoV-2 spike glycoprotein and ORF8 in the course of COVID-19

Abstract Immunoassays are a standard diagnostic tool that assesses immunity in severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) infection. However, immunoassays do not provide information about contaminating antigens or cross-reactions and might exhibit inaccurately high sensitivity low specificity. We aimed to gain insight into the serological immune response of SARS-CoV-2 patients by immunoblot analysis. analyzed serum immunoglobulins IgM, -A, -G directed against proteins analysis from 12 infected patients. determined IgG isotype antibodies commercially available ELISA assessed clinical parameters inflammation status kidney liver injury. Unexpectedly, we found no correlation between presence future course disease. attention should be paid CRP, IL-6, LDH. evidence antibody cross-reactivity, which questions reliability results for samples tested negative anti-SARS-CoV-2 when immunoassays. Nevertheless, detection antibodies, our data suggest use spike glycoprotein sufficient identify positive Using combination open reading frame 8 protein could prove best way detecting IgM antibodies.